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Washing machine And after deprotection is finished, adding 50ml of DMF (dimethyl formamide) washing resin into the resin, draining, adding DMF again for washing, repeating the washing for 6 times in total, and taking the resin ninhydrin for positive detection

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This occurs when thermal stress disrupts tertiary structure without fully denaturing the peptide—the molecule remains soluble but loses its active conformation