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Emerging tools such as real-time biosensors and optogenetic approaches, super-resolution microscopy, including in situ structural biology methods such as cryo-electron tomography (cryo-ET) and cryo-correlative light and electron microscopy (cryo-CLEM), as well as spatial proteomics and the above-mentioned proximity labeling approaches offer unprecedented opportunities to map the assembly, localization, and kinetics of individual receptor-associated signaling complexes
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