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Organoid or monolayers were then incubated overnight at 4 °Cwith primary antibodies for CFTR (Merk, MAB3480), ZO-1 (Invitrogen, 40-2200) and FABP (Abcam, ab7366), followed by secondary antibody staining at room temperature for 2 h

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When the customer picks out the items they’d like, they are guided to a medical questionnaire that a physician will look over and help them with health and longevity goals