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Berberine as a multi-target therapeutic agent for obesity: from pharmacological mechanisms to clinical evidence | European Journal of Medical Research | Springer Nature Link
Exponential growth phase cells were transfected with 2.5 μg shPPARα in reduced serum medium (OPTI-MEM-I) according to the manufacturer’s protocol at 50–70% confluence
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doi: 10.2215/CJN.04010413
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