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Tissue culture media supernatants from the final 24 hours of culture were collected and assayed by Amplex Red Glucose/Glucose Oxidase Assay Kit (Life Technologies, Gaithersburg, MD, USA), Lactate Assay kit (Sigma-Aldrich) and Rat Ultrasensitive Insulin ELISA (Mercodia, Uppsala, Sweden) to measure total glucose, lactate and insulin respectively
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