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SupersmartTM2 × ZAPA3G SYBR Green qPCR Mix (Zsgentech, Tianjin, China) and cobas® z 480 Automatic Fluorescence Quantitative PCR analyzer (Roche, Germany) were used for RT-qPCR, and the reaction conditions were set as follows: at 95 ℃ for 5 min, 40 cycles at 95 ℃ for 10 s, at 60℃ for 20 s, and at 70 ℃ for 10 s
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Elevated 2-AG acts as a metabolic handbrake, calming hyperactive, stress-driven neural circuits in the brain
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